C4d staining of renal allograft biopsies: a comparative analysis of different staining techniques

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C4d staining of renal allograft biopsies: a comparative analysis of different staining techniques.

BACKGROUND Detection of C4d along peritubular capillaries (PTC) in renal allograft biopsies is an independent prognostic marker of poor long-term graft survival. It is typically associated with circulating donor-specific antibodies. Since only little information is available on the best technique to stain C4d, we compared the two methods most often used for detecting C4d in renal allograft spec...

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Immunostaining of renal allograft biopsies for C4d deposition has become an important diagnostic tool in the recognition of humoral-mediated graft rejection. The majority of studies have been performed on frozen tissue sections with one of several commercially available antibody reagents. However, only a single small series that compared reagents or methods, including staining of formalin-fixed...

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ژورنال

عنوان ژورنال: Nephrology Dialysis Transplantation

سال: 2006

ISSN: 0931-0509,1460-2385

DOI: 10.1093/ndt/gfl594